Arcturus Cellect LCM system
Laser microdissection is a well-established method for isolating specific tissue areas or cell populations from tissue specimen or heterogeneous cell populations either FFPE or fresh-froszen tissue sections. The Arcturus System combines laser-capture and laser cutting in one device by offering two lasers (UV, IR). This offers flexibility in preparation, allowing you to use any slide type. The use of the IR laser is ideal for capturing single cells and/or small numbers of cells.
Areas of interest are identified under the microscope (using 2x, 4x, 10x, 20x, 40x, 60x objectives) and isolated using a combination of laser-capture and laser cutting with two lasers (UV and IR). The system includes LED brightfield, Phase Contrast, and LED Epi-Fluorescence Illumination.
If you are interested in using the Arcturus Cellect LCM System, please contact Enni Markkanenenni.markkanen@vetpharm.uzh.ch for details.
Literature and Links
Seiteninhalt
Location
University Zurich, Institute of Veterinary Pathology, Room TDI-020
Responsible Persons & Training Requests
For any questions about the microscope please contact Alexandra Malbon. Email: alexandra.malbon@uzh.ch
Technical Specifications
Microscope
- inverted microscope (Nikon Eclipse Ti-E)
- UV cutting laser
- IR laser for capturing (LCM)
- stage insert for three 75mm x 25 mm slides
Light Sources
- Halogen lamp for transmitted light
- Fluorescence lamp - metal halide lamp
Objectives
| Name | Magnification | NA | Immersion | WD (mm) |
|---|---|---|---|---|
| CFI Plan Apo | 2x | 0.06 | Air | 7.5 |
| CFI PlanFluor | 10x (Ph1) | 0.3 | Air | 15.2 |
| CFI S PlanFluor ELWD ADM | 20x (Ph1) | 0.45 | Air | 6.9-8.2 |
| CFI S PlanFluor ADM ELWD | 40x (Ph2) | 0.6 | Air | 2.8-3.6 |
| CFI S PlanFluor ELWD ADL | 60x (Ph2) | 0.7 | Air | 1.8-2.62 |
Fluorescence Filters
| Name | Excitation Range | Excitation Filter | Dichroic | Emission Filter |
|---|---|---|---|---|
| DAPI | UV | BP 385/20 | 410 | BP 445/60 |
| eGFP | blue | BP 470/40 | 500 | BP 520/40 |
| TxRed | green | BP 562/45 | 590 | BP 625/50 |
| Triple | UV/blue/green | BP 410/20; 495/30; 575/30 | 445; 515; 610; | BP 455/30; 530/30; 625/40 |
Camera Systems
1024 x 768 1/3” color CCD with electronic shutter to 1/10000 s and on-chip integration to 30 seconds
Accessories
- Heating/cooling block