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Institut für Veterinärpathologie

Specific technical services and equipment / Spezifische technische Services und Geräte

Slide Scanning Service for Histology, Cytology, Immunohistology and Immunofluorescence

Virtual slides are increasingly used in research and teaching, they can also serve as an effective tool to archive histological specimens.

The IVPZ offers a range of slide scanning services that fit various needs. We host a high-end slide scanner (Nanozoomer HT 2.O, Hamamatsu) that is equipped for high quality scanning of cytological and histological specimens (HE and special stains) including immunohistology and immunofluorescence. The system is suitable for a range of commercial fluorophores: https://www.semrock.com/SetDetails.aspx?id=3302 (see under “Fluorophore”).

Digital slides from stained sections can be provided within a few days, through one of the following two options:

  • Files are saved on your own external drive.
  • Flies are transferred to you via an ftp-server and can be downloaded to your own computer.

Charges

For UZH scientists

The basic price for scanning in bright field is 5 CHF per scan, for the scanning of immunofluorescence slides it is 10 CHF per scan. For immunofluorescence, an additional fee of 50 CHF applies, for the initial optimisation of the scanning parameters. Please note that an overhead of 20% might apply.

For external clients

The basic price for scanning in bright field is 10 CHF per scan, for the scanning of immunofluorescence slides it is 15 CHF per scan. The initial optimisation of the scanning parameters for immunofluorescence is 70 CHF.

For the scanning of larger sets of slides a price reduction can be applied.

Scanning in combination with uploading on a server for teaching use: please contact Prof. Franco Guscetti (gufo@vetpath.uzh.ch).

If you are interested in this service or have questions about it, please contact Prof. Franco Guscetti gufo@vetpath.uzh.ch or the Laboratory for Animal Model Pathology (LAMP ivpzlamp@vetpath.uzh.ch); phone: 044 635 85 95.

Histomorphometry and digital slide analysis

Our morphometry software (Visiomorph, from Visiopharm) provides a professional solution for the automated quantitative scoring of conventionally stained histopathological slides as well as of immunohistochemical and immunofluorescence stains. The software can be used both on microphotographs and on whole digital slides.

Pricing

If you are interested in our histomorphometrical analysis service which is conducted by a qualified pathologist, please contact LAMP (ivpzlamp@vetpath.uzh.ch) for a quotation.

Alternatively, the researcher can work independently at one of the Visiomorph workstations at our Institute.

For UZH members

The use of the Visiomorph software can be booked at the basic price of 10 CHF per hour.

In addition, a one-off fee of 175 CHF applies for a one hour introduction to the specific use of the software by a qualified pathologist. It also includes help in developing the relevant apps and continuous support by the pathologist.

For external clients

The use of the Visiomorph software can be booked at the basic price of 20 CHF per hour.

In addition, a one-off fee of 175 CHF applies for a one hour introduction to the specific use of the software by a qualified pathologist. It also includes help in developing the relevant apps and continuous support by the pathologist.

If you are interested in this service or have questions about it, please contact Prof. Franco Guscetti (gufo@vetpath.uzh.ch) or the Laboratory for Animal Model Pathology (LAMP, ivpzlamp@vetpath.uzh.ch).

Transmission electron microscopy and ultrastructural pathology

The Institute hosts a dedicated Electron Microscopy Unit staffed with expert technicians and headed by an ultrastructural pathologist. The Unit provides the full range of technical services required for transmission electron microscopy (TEM), ranging from fixation over embedding, the preparation of semithin and thin section, to the provision of digital images from both semithin and thin sections. It is also experienced in immune-TEM and the processing of samples for 3D-TEM.

If you are interested in this service or have questions about it, please contact the Dr. Udo Hetzel via email or call 044 635 85 61.

Arcturus Cellect LCM system

Laser Microdissection ArcturusXT

Laser microdissection is a well-established method for isolating specific tissue areas or cell populations from tissue specimen or heterogeneous cell populations either FFPE or fresh-froszen tissue sections. The Arcturus System combines laser-capture and laser cutting in one device by offering two lasers (UV, IR). This offers flexibility in preparation, allowing you to use any slide type. The use of the IR laser is ideal for capturing single cells and/or small numbers of cells.

Areas of interest are identified under the microscope (using 2x, 4x, 10x, 20x, 40x, 60x objectives) and isolated using a combination of laser-capture and laser cutting with two lasers (UV and IR). The system includes LED brightfield, Phase Contrast, and LED Epi-Fluorescence Illumination.

If you are interested in using the Arcturus Cellect LCM System, please contact Enni Markkanenenni.markkanen@vetpharm.uzh.ch for details.

Literature and Link

Technischer Service für Histologielabore

Das Histologielabor des IVPZ bietet Forscher*innen und Forschergruppen sowie anderen Laboren einen technischen Service an, der von der Aufarbeitung frischen oder fixierten Materials (Gewebe, Zellkulturmaterial, Schnitte etc.) für die Histologie zur Präparation gefärbter Schnitte reicht. Hierzu gehört auch die Anwendung von bereits etablierten immunhistologischen Färbungen und von  Immunfluoreszenz-Protokollen, das Austesten neuer Antikörper für die Immunhistologie und Immunfluoreszenz sowie die Etablierung neuer Färbeprotokolle.   

Falls Sie an unserem technischen Service interessiert sind, kontaktieren Sie uns bitte per Email oder per Telefon: 044 635 85 61

Weiterführende Informationen

Contacts / Kontakte

Slide Scanning Service
Email Prof. Dr. Franco Guscetti or
Laboratory for Animal Model Pathology;
phone: 044 635 85 95

Histomorphometry and digital slide analysis
Email Prof. Dr. Franco Guscetti or
Laboratory for Animal Model Pathology

Contacts / Kontakte

Transmission electron microscopy and ultrastructural pathology
Email Dr. Udo Hetzel;
phone: 044 635 85 61

Arcturus Cellect LCM system
Email enni.markkanen@vetpharm.uzh.ch

 

Contacts / Kontakte

Technischer Service für Histologielabore
Email ivpz@vetpath.uzh.ch;
Tel.: 044 635 85 91 / 044 635 85 50