Specific technical services and equipment / Spezifische technische Services und Geräte

Slide Scanning Service for Histology, Cytology, Immunohistology and Immunofluorescence

Virtual slides are increasingly used in research and teaching, they can also serve as an effective tool to archive histological specimens.

The IVPZ offers a range of slide scanning services that fit various needs. We host a high-end slide scanner (Nanozoomer HT 2.O, Hamamatsu) that is equipped for high quality scanning of cytological and histological specimens (HE and special stains) including immunohistology and immunofluorescence. The system is suitable for a range of commercial fluorophores: https://www.semrock.com/SetDetails.aspx?id=3302 (see under “Fluorophore”).

 

Digital slides from stained sections can be provided within a few days, through one of the following two options:

  • Files are saved on your own external drive.
  • Flies are transferred to you via an ftp-server and can be downloaded to your own computer.

 

Charges:

For UZH scientists:

The basic price for scanning in bright field is 5 CHF per scan, for the scanning of immunofluorescence slides it is 10 CHF per scan. For immunofluorescence, an additional fee of 50 CHF applies, for the initial optimisation of the scanning parameters. Please note that an overhead of 20% might apply.

 

For external clients:

The basic price for scanning in bright field is 10 CHF per scan, for the scanning of immunofluorescence slides it is 15 CHF per scan. The initial optimisation of the scanning parameters for immunofluorescence is 70 CHF.

 

For the scanning of larger sets of slides a price reduction can be applied.

 

Scanning in combination with uploading on a server for teaching use: please contact Prof. Franco Guscetti (gufo@vetpath.uzh.ch).

 

If you are interested in this service or have questions about it, please contact Prof. Franco Guscetti (gufo@vetpath.uzh.ch) or the Laboratory for Animal Model Pathology (LAMP, ivpzlamp@vetpath.uzh.ch); phone: 044 63 58 595

Histomorphometry and digital slide analysis

Our morphometry software (Visiomorph, from Visiopharm) provides a professional solution for the automated quantitative scoring of conventionally stained histopathological slides as well as of immunohistochemical and immunofluorescence stains. The software can be used both on microphotographs and on whole digital slides.

 

Pricing:  

If you are interested in our histomorphometrical analysis service which is conducted by a qualified pathologist, please contact LAMP (ivpzlamp@vetpath.uzh.ch) for a quotation.

Alternatively, the researcher can work independently at one of the Visiomorph workstations at our Institute.

For UZH members:

The use of the Visiomorph software can be booked at the basic price of 10 CHF per hour.

In addition, a one-off fee of 175 CHF applies for a one hour introduction to the specific use of the software by a qualified pathologist. It also includes help in developing the relevant apps and continuous support by the pathologist.

 

For external clients:

The use of the Visiomorph software can be booked at the basic price of 20 CHF per hour.

In addition, a one-off fee of 175 CHF applies for a one hour introduction to the specific use of the software by a qualified pathologist. It also includes help in developing the relevant apps and continuous support by the pathologist.

If you are interested in this service or have questions about it, please contact Prof. Franco Guscetti (gufo@vetpath.uzh.ch) or the Laboratory for Animal Model Pathology (LAMP, ivpzlamp@vetpath.uzh.ch); phone: 044 63 58 558

Transmission electron microscopy and ultrastructural pathology

The Institute hosts a dedicated Electron Microscopy Unit staffed with expert technicians and headed by an ultrastructural pathologist. The Unit provides the full range of technical services required for transmission electron microscopy (TEM), ranging from fixation over embedding, the preparation of semithin and thin section, to the provision of digital images from both semithin and thin sections. It is also experienced in immune-TEM and the processing of samples for 3D-TEM.

If you are interested in this service or have questions about it, please contact the Dr. Udo Hetzel via email or call 044 63 58 561.

Laser Microdissection ArcturusXT

 

Laser Microdissection ArcturusXT

Laser microdissection is a well-established method for isolating individual or subcellular structures from heterogeneous cell populations. The Arcturus System combines laser-capture and laser cutting in one device by offering two lasers (UV, IR). This offers flexibility in preparation, allowing you to use any slide type. The use of the IR laser is ideal for capturing single cells and/or small numbers of cells.

Location

University Zurich, Institute of Veterinary Pathology, Room TDI-020

 

Responsible Persons & Training Requests / Reservation

For any questions about the microscope please contact Josep Monné Rodríguez: email: josep.monnerodriguez@uzh.ch; phone: 044 63 58 597

If you are interested in using the Laser Microdissection Arcturus XT for the first time please contact us for an initial training session (free of charge, afterwards 10 CHF/hour). All users need to be registered with the system in order to subsequently reserve the machine (a prior ZMB registration also gives access); please follow this link if you are not already registered.  Registered users can link to the reservation system.

 

Technical Specifications

Microscope

  • inverted microscope (Nikon Eclipse Ti-E)
  • UV cutting laser
  • IR laser for capturing (LCM)
  • stage insert for three 75mm x 25 mm slides 

Light Sources

  • Halogen lamp for transmitted light
  • Fluorescence lamp - metal halide lamp

 

Objectives

Name Magnification NA Immersion WD (mm)
CFI Plan Apo 2x 0.06 Air 7.5
CFI PlanFluor 10x (Ph1) 0.3 Air 15.2
CFI S PlanFluor ELWD ADM 20x (Ph1) 0.45 Air 6.9-8.2
CFI S PlanFluor ADM ELWD 40x (Ph2) 0.6 Air 2.8-3.6
CFI S PlanFluor ELWD ADL 60x (Ph2) 0.7 Air 1.8-2.62

Fluorescence Filters

Name Excitation Range Excitation Filter Dichroic Emission Filter
DAPI UV BP 385/20 410 BP 445/60
eGFP blue BP 470/40 500 BP 520/40
TxRed green BP 562/45 590 BP 625/50
Triple UV/blue/green    BP 410/20; 495/30; 575/30 445; 515; 610; BP 455/30; 530/30; 625/40

Camera Systems

1024 x 768 1/3” color CCD with electronic shutter to 1/10000 s and on-chip integration to 30 seconds

Accessories

  • Heating/cooling block
 
 
 

Materials - ordering Information

 

Technischer Service für Histologielabore

Das Histologielabor des IVPZ bietet Forschern und Forschergruppen sowie anderen Laboren einen technischen Service an, der von der Aufarbeitung frischen oder fixierten Materials (Gewebe, Zellkulturmaterial, Schnitte etc.) für die Histologie zur Präparation gefärbter Schnitte reicht. Hierzu gehört auch die Anwendung von bereits etablierten immunhistologischen Färbungen und von  Immunfluoreszenz-Protokollen, das Austesten neuer Antikörper für die Immunhistologie und Immunfluoreszenz sowie die Etablierung neuer Färbeprotokolle.   

Falls Sie an unserem technischen Service interessiert sind, kontaktieren Sie uns bitte per Email oder per Telefon: 044 63 58 561