Laser Microdissection ArcturusXT

Laser Microdissection ArcturusXT

Laser microdissection is a well-established method for isolating individual or subcellular structures from heterogeneous cell populations. The Arcturus System combines laser-capture and laser cutting in one device by offering two lasers (UV, IR). This offers flexibility in preparation, allowing you to use any slide type. The use of the IR laser is ideal for capturing single cells and/or small numbers of cells.

Location

University Zurich, Institute of Veterinary Pathology, Room TDI-020

Responsible Persons & Training Requests

For any questions about the microscope please contact Alexandra Malbon. Email: alexandra.malbon@uzh.ch

Technical Specifications

Microscope

  • inverted microscope (Nikon Eclipse Ti-E)
  • UV cutting laser
  • IR laser for capturing (LCM)
  • stage insert for three 75mm x 25 mm slides 

Light Sources

  • Halogen lamp for transmitted light
  • Fluorescence lamp - metal halide lamp

Objectives

Name Magnification NA Immersion WD (mm)
CFI Plan Apo 2x 0.06 Air 7.5
CFI PlanFluor 10x (Ph1) 0.3 Air 15.2
CFI S PlanFluor ELWD ADM 20x (Ph1) 0.45 Air 6.9-8.2
CFI S PlanFluor ADM ELWD 40x (Ph2) 0.6 Air 2.8-3.6
CFI S PlanFluor ELWD ADL 60x (Ph2) 0.7 Air 1.8-2.62

Fluorescence Filters

Name Excitation Range Excitation Filter Dichroic Emission Filter
DAPI UV BP 385/20 410 BP 445/60
eGFP blue BP 470/40 500 BP 520/40
TxRed green BP 562/45 590 BP 625/50
Triple UV/blue/green    BP 410/20; 495/30; 575/30 445; 515; 610; BP 455/30; 530/30; 625/40

Camera Systems

1024 x 768 1/3” color CCD with electronic shutter to 1/10000 s and on-chip integration to 30 seconds

Accessories

  • Heating/cooling block